Use of blood specimens collected on filter paper in screening for abnormal hemoglobins.

[Neonatal screening for hemoglobinopathies: a pilot study in Porto Alegre, Rio Grande do Sul, Brazil].

This study was conducted to establish the frequency of hemoglobinopathies among newborns undergoing screening tests for metabolic diseases at the University Hospital (Hospital de Clínicas) in Porto Alegre, Rio Grande do Sul, Brazil. Testing for abnormal hemoglobins was performed by isoelectric focusing electrophoresis on agarose gel with blood obtained by heel stick and applied to filter paper....

Cord blood screening for hemoglobin disorders by high-performance liquid chromatography.

Ion-exchange high-performance liquid chromatography was employed as a screening method for abnormal hemoglobins in the newborn period. Samples of cord blood collected in EDTA tubes were used for this analysis. Hemolysates were injected onto 4.1 X loo-mm Synchropak ion-exchange columns using an automatic injector. Hemoglobin separation was carried out by means of a sodium acetate gradient. A tot...

The use of dried whole blood absorbed on filter-paper for the Evaluation of Diphtheria and Tetanus Antitoxins in Mass Surveys

Detection of Alpha Thalasemia in Cord Blood in Bahawalpur Region

Background: Neonatal screening for abnormal hemoglobins provides a practical approach for the detection of sickling syndromes and other hemoglobin disorders such as a-thalassemia. Most screening programs utilize various electrophoretic techniques for the detection of abnormal hemoglobins Electrophoresis on cellulose acetate at pH 8.6 identifies increased amounts of Bart’s hemoglobin (Hb) associ...

f 3 - LipoproteinQuantitationin CordBloodSpottedon FilterPaper : A

We describe an electroimmunodiffusion technique for measuring /3-lipoprotein in cord blood spotted on filter paper. A series of cord-blood samples, taken from 916 consecutive live-birth infants, was spotted directly onto filter paper and assayed for /3-lipoprotein. Eleven had abovenormal /3-lipoprotein. Of these 11, seven were tested two to six months later, along with their parents, for total ...